The Hardy Weinberg Theorem In Genetics Biology Essay

The Hardy Weinberg Theorem In Genetics Biology Essay Introduction The Hardy Weinberg Theorem is a mathematical formula that allows allele and genotype frequencies in a population of diploid or polypoid individuals to be interrelated, where the frequency of one allele is represented as p, and the frequency of the other is represented as q (the sum of which = 1.0). The sum of the different genotype frequencies (homozygotes and heterozygotes) also equates to 1.0. Where p and q are the frequencies of the alleles for a particular gene in a population, the genotype frequency can be expressed as: p ² + 2pq + q ² = 1 Where p ² = frequency of organisms that are homozygous for the first allele q ² = frequency of organisms that are homozygous for the second allele 2pq = frequency of heterozygous organisms The Hardy Weinberg equilibrium stays constant as long as there is random mating, no migration, no natural selection, no mutation and no genetic drift (N=infinite), (Fig. 1). N = infinite Figure 1. A graph to show the Hardy Weinberg Equilibrium. Put together using data from: Lori Lawsons lecture 15, Neutral Evolution and Genetic Drift, 2010 Therefore the population does not evolve. If an allele or genotype frequency is seen to change from one generation to the next then it is clear that one or more of the micro-evolutionary forces (mutation, migration etc) are acting on those traits in the population. Without mutation there are no new alleles or genes and so no evolution. Mutation must occur in the germ line to be significant in evolutionary terms. J. B. S. Haldane (1892-1964) stated that the number of germ cell divisions per generation is higher in males; therefore the mutation rate will be higher in males. Gene flow (also called migration) brings new genotypes into populations and is critical for the long term survival of a population, especially if it is a small population. For migration to be Catherine Carrick 200884273 effective in respect to evolution there must be successful reproduction among migrating populations. It is the movement of alleles between populations, not individuals. Wrights Island model of migration (Fig. 2) shows that migration homogenizes populations (where they consist of similar elements uniform throughout). Genetic drift is another form of micro-evolution and leads to random changes in allele frequencies. It is fundamentally a result of finite population size and has the most rapid and dramatic effect on small populations who show reduced variability. Drift increases divergence between populations so genetic variation must be replenished. Mutation replenishes variation and at equilibrium there is a balance between the rate of mutation and the rate of drift. Figure 2.Wrights island model. Put together using data from: Lori Lawsons lecture 15, Neutral Evolution and Genetic Drift, 2010 Charles Darwin (1859) defined natural selection (another micro-evolutionary force) with his four postulates; 1) individuals within populations are variable, 2) there is heritability (variation among individuals partly passed on from parents), 3) that in every generation there are some individuals that are fitter (survival/reproductive success) than others and 4) fitness is not random. Natural selection is the equivalent of differential reproduction as a result of an organisms interaction with the environment and the populations inherent variation. It acts on heritable (not acquired) characteristics at an individual level and not for the good of the species. However, the consequences occur in populations. This is demonstrated by melanism in the peppered moth (Biston betularia). The causes of melanism in the peppered moth have been well studied since the 1950s and show natural selection at work. Camouflage is key to predator avoidance in the peppered moth and there are two distinct mor phs. One being white with black or brown specks (typica) and the other predominantly black (carbonaria). The former is well camouflaged on trees with lichen on their bark and the later; better suited to dark or blackened bark. During the industrial revolution in the 19th century, an increase of soot and industrial pollution coincided with the appearance of the carbonaria form. Original studies on the relation between B. betularias crypsis and lichens failed to consider two important details; firstly, that the natural resting place of the moths is high in the canopy during the day and not on the trunk as previously thought, and secondly, human vision was used to simulate a birds view of the moths originally, but avian species are sensitive to different wavelengths of light and so will have a different view of the moths and their respective camouflage to that of humans. Taking this into consideration, Majerus, Brunton and Stalker (2000) devised a more systematic experiment to examine the UV characteristics of Catherine Carrick 200884273 both moth morphs and some of the lichens they rested on as demonstrated by the images in Fig. 3. Figure 3. The typica and carbonaria forms of the peppered moth as they appear in normal visible light (a), and as they appear under UV light (b). Image taken from MAJERUS, BRUNTON STALKER, 2000 It was their conclusion that moth colour provides sufficient camouflage both in human-visible and UV- spectra to crutose lichens (as appose to different lichen flora originally hypothesised to be rested upon by black and peppered morphs). Ultimately, strong selection pressures gave way to relatively rapid the evolution of the carbonaria form in industrialised areas due to the advantages of its dark colour (predator avoidance etc). Methods and Results Assignment 1: Testing the Hardy-Weinberg Principle: Method Using PopGenLab, we are able to set up hypothetical experiments to observe the factors that influence the Hardy Weinberg equilibrium in a population. We can do this by manipulating different input parameters (genotype frequency, tree type, number of stands (groups of trees), stand size (number of trees within a group), migration rate, mating pattern and disaster frequency). For this assignment the input parameters are as follows: Number of stands = 1 All other input parameters are left at default values (equal allele frequencies; genotype frequencies of 50% brown, 25% white, 25% black; equal proportions of each tree type; stand size of 4000; no migration; random mating; disaster frequency set at Never. Results Q 1.1) When looking at the allele and genotype frequencies, there is a change in both over time. All populations behave differently to one another. This is because the only active evolutionary force is genetic drift. Fig. 4 shows that allele frequencies change Catherine Carrick 200884273 over time due to genetic drift, but as all the conditions of the Hardy Weinberg equilibrium are fixed the allele frequencies must equal 1 and so the variation in allele frequency of A becomes the negative of the frequency of a. (Fig. 4). Figure 4. Showing allele frequencies changing over time due to genetic drift. Blue line = Allele A, Red line = Allele a, Green line = average over all stands for allele A Q 1.2) When the initial allele frequencies are changed to A=80% (p), a = 20% (q) (p ² = 0.8 x 0.8 = 0.64); AA = 64% (equilibrium reached after one generation) (Fig 6). If all the Hardy Weinberg conditions are all fixed, the equilibrium will always be reduced in the next generation (Fig.5 and 6). Fig. 5. shows the initial genotype frequency compared with Fig. 6. which shows the genotype frequency after one generation. The actual genotype frequencies (worked out with average stand number) match the Hardy Weinberg predictions as they stay within 1% of the previous generations genotype frequency, across every generation thereafter. However, the percentage may change by 1% due to genetic drift. Figure 5. Display of the initial genotype frequency. Catherine Carrick 200884273 Figure 6. Shows the Hardy Weinberg equilibrium is reached after one generation where 0.64=64% homozygous AA individuals. Assignment 2: Genetic Drift Method Q 2.1) Firstly we ran an experiment with default values for all the Hardy Weinberg conditions and 100 populations. We then ran a series of experiments with 100 populations and default parameters for all conditions except tree stand size which was systematically reduced for each experiment. We recorded the effects on allele and genotype frequency (below). Fig. 7 shows that stand size 10 produced the largest fluctuations of allele frequencies, and displayed the most cases of allele fixing. Results Stand size = 4000 (carrying capacity) Allele frequency- the average remains constant for A and a Genotype frequency stays relatively constant throughout. Stand size = 2000 Allele frequency the average show slight variation Genotype frequency stays relatively constant. Stand size = 1000 Allele frequency the average starts to diverge more with each generation from F45 (generation 45) onwards showing a lot more variation than in larger stand sizes Genotype frequency the average frequency stays constant although there is some variation compared with larger stand sizes Catherine Carrick 200884273 Stand size = 500 Allele frequency The average shows variation in the later generations Genotype frequency the average stays relatively constant but still with more variation than in any other larger stand thus far Stand size = 250 Allele frequency the average shows some variation in the mid-generations, but this returns to a 50:50 frequency in the later generations Genotype frequency on average, the frequency of both white and black variations of moth increases and shows a large variation between stands. Heterozygosity the brown variation decreases by 9% over 100 generations Stand size = 100 Allele frequency the average shows more variation, but to the point where in some stands alleles within individual populations become fixed Genotype frequency the frequency of homozygotes increases. In some stands the homozygosity (black) becomes fixed, phasing out the other tow variations (white and brown) Heterozygosity drops by 22% Stand size = 50 Allele frequency frequencies become fixed for a single allele quickly (by F23) Genotype frequency Many stands become fixed for one variation within a few generations Heterozygosity drops by 33% after 100 generations Stand size = 10 Allele frequency becomes fixed within a population after two generations, and continues to become fixed in other populations. By F77, all are fixed Genotype frequency every single genotype becomes homozygous or extinct Heterozygosity by F77 all heterozygosity is lost and by F100 there are only homozygous populations, with the other (70%) becoming extinct There are many variations in allele and genotype frequency between different stands because as the stand number decreases, the chance of genetic drift increases. Figure 7 shows that at stand size 10, heterozygosity was lost completely by F77. Catherine Carrick 200884273 Figure 7. (stand size 10): this produced the largest fluctuations of allele frequencies, and displayed the most cases of allele fixing. (Blue = A, Red = a, Green = average) Q 2.2) As the stand size decreased, so did the heterozygosity. Populations began fixation in stand size 100 to stand size 50. As the stand size decreased, the number of fixed alleles increased. When the carrying capacity became too small, there was not enough variation to prevent fixation. Random mating account for the variation between fixed and non-fixed alleles in stand size 100 and stand size 50. Fig. 8. shows that with a stand size of 10, heterozygosity diminished completely by F77. Therefore, the smaller the population, the quicker heterozygosity is lost. Figure 8. Question 2:2 (stand size 10): This shows the heterozygosity diminished completely by F77. The green line (average) tends to diminishing heterozygosity. Catherine Carrick 200884273 Figure 9. Question 2:3 (stand size 10): Shows population of stand number 15 and how it fluctuates around the average value, it also shows that when the population dwindled to a certain point, it wasnt able to re-establish the numbers enough to prevent extinction. Q 2.3) Yes populations from stand size 10 became extinct (70% of them) therefore, as the carrying capacity decreases, the risk of extinction increases. There is variation within generations due to factors like predation or whether the offspring are male biased for example. There may be a lower population size in the next generation depending on mating strategies (random mating) and occasionally, the parameters reach a point of no return and the population can not recover and so becomes extinct. Others avoid extinction because the experiment is random. Fig. 9. (where stand size = 10) shows population of stand number 15 and how it fluctuates around the average value, it also shows that when the population dwindled to a certain point, it wasnt able to re-establish the numbers enough to prevent extinction. Assignment 3: The Influence of Mating Patterns on Population Genetics Method In this experiment we set all default parameters except for the number of tree stands which was set to 100. The first experiment was carried out with random mating, and the subsequent experiments with non-random mating. We then varied the population size as before, this time to compare the effects of assertive mating with genetic drift. Results Q 3.1) The effects of 25% assortative mating: Genotype frequency 25% assortative mating causes an increase in homozygotes, and heterozygosity is lost by F80 Allele frequency (produces a sigmoidal shaped graph). All become fixed for a single allele. Heterozygosity the average heterozygosity is lost at F80 Catherine Carrick 200884273 50% assortative mating: Genotype frequency all homozygotes with an almost 1:1 ratio aa being slightly more dominant Allele frequency (sigmoidal graph) all fixed by F50 Heterozygosity lost by F33 (average heterozygosity) 100% assortative mating Genotype frequency quickly becomes homozygote dominated Allele frequency All fixed fro a single allele by F15 Heterozygosity Average lost by F4 Heterozygosity is lost under assortative mating. This is because heterozygotes are at a reproductive disadvantage as homozygotes will mate with like genotypes. Heterozygotes will not be produced by these matings either. Q 3.2) Results Population size 2000 (stand size), 100% assortative mating: Genotype frequency all homozygous by F4 Allele f fixed by F14 Heterozygosity average lost by F4 Population size 2000, 50% assortative mating Genotype f all homozygous by F25 Allele f all fixed by F29 Heterozygosity average lost by F25 Population size 250, 100% assortative mating Genotype f all homozygous by F4 Allele f all fixed by F12 Heterozygosity lost at F4 Population size 250, 50% assortative mating Genotype f all homozygous by F25 Allele f all fixed by F28 Heterozygosity lost at F25 Assortative mating dominates control of allele frequencies and the speed that alleles become fixed within a population compared with the effects of genetic drift (because the homozygotes are all mating with the same genotype and not with heterozygotes). Assortative mating is not dependant on carrying capacity. The size of the population is irrelevant when assortative mating is occurring. The results are similar for a high or a low population size. Catherine Carrick 200884273 Q 3.3) Method We conducted a series of experiments using disassortative mating and selected different levels of mating between 0% (random mating) and 100% (only unlike phenotypes mate). We then changed the population size from 2000 to 250 to see the effects of disassortative mating on genetic drift. Results Dissasortative mating shows that AA and aa will mate which increases heterozygosity and stabilises the population as shown in the results below: Population size 2000, 100% disassortative mating Geno (genotype frequency) heterozygote is predominant Allele (allele frequency) none become fixed. There is variation but it stays within 31% 68% variation Hetero (heterozygosity) increases in the first generation then remains constant Pop size 2000, 50% disassortative mating Geno predominantly heterozygote Allele none become fixed. There is less variation than with 100% disassortative mating. Variation is between 43% and 57% Hetero Increases in 1st generation and remains constant Pop size 250, 100% disassortative mating Geno -slight heterozygote increase Allele No fixed alleles. There is much greater variation than seen previously with a larger population size, between 21% and 79% Hetero increases in 1st generation then remains steady and begins to decrease. Remains above the initial percentage Pop size 250, 50% disassortative mating Geno heterozygosity increases steadily Allele No fixed alleles. Variation is less than with 100% disassortative mating and population size of 250. Variation levels out between 33% and 67% Hetero increases in the 1st generation and remains constant with a few small fluctuations which level back out Q 3.4) There would be more heterozygosity in the next generation when disassortative mating occurs and if this kind of mating is maintained, the effects of genetic drift occur much slower because the populations are prevented from diverging. Fig. 10 shows the comparison between disassortative mating and random mating where random mating allows genetic drift. Drift can still occur during dissasortative mating when the carrying capacity is very low. Catherine Carrick 200884273 Figure 10. (picture on left): Random mating, pop size 250 showing genetic drift acting to diverge allele frequencies. (picture on right): 50% dissassortative mating, population size 250 shows that dissasortative mating acts to counter genetic drift. Q 3.5) Method For this experiment we varied the initial genotype frequency for assortative and disassortative mating. We tried experiments where the initial allele frequency favoured one or the other allele. Fig. 11 show starting frequencies of 50/50% assortative mating. A small deviation in starting frequencies affects the final fixation percentages (Fig. 11). We did not include the brown allele in this experiment as the extra variable is not needed. Figure 11) Shows starting frequencies of 50/50% (50%-white allele, 50% black allele) with assortative mating = 100%. Small deviation in starting freq effects final fixation percentages. Catherine Carrick 200884273 Results Under dissassortative mating the time taken for equilibrium to establish is negatively correlated with the degree of deviation from a 1:1 starting allele ratio. Under assortative mating, fixation or loss of alleles is negatively correlated with the degree of deviation from a 1:1 starting allele ratio. Assignment 4: Modes of Natural Selection Q 4.1) Method In this experiment we investigated how fitness affects changes in allele frequency in the population. We began with default parameters except tree stand number (set at 100) and genotype frequencies. We changed the tree frequency to set up several experiments under conditions of directional selection for dark moths, directional selection for light moths, balancing selection favouring the brown moth, and diversifying selection favouring the dark and light moths. We tried experiments with the different conditions of selection and initial allele frequencies near zero and one. Results Directional selection for black moths where they tree frequencies are 50% black, 25% white and 25% brown trees gave the following results: Allele frequency becomes fixed rapidly by F10 (on average by F9) Genotype frequencies at F10 genotype becomes fixed for black allele In a small population, alleles become fixed more quickly but in larger populations allele frequencies are not affected as much. We kept the population size high so we would not see genetic drift in the experiment (4000 carrying capacity) with tree frequencies of 35%, 32% and 33%. Even the small amount of selection (35% black trees) shows fixation of alleles for the black morph of moth (Fig. 12). Selection for light moths gives the same results as selection for black moths. Figure 12. shows allele becoming fixed rapidly, due to a tiny increase in black trees on left, white trees on right (35 %) Catherine Carrick 200884273 Q 4.2) Starting figures are as follows: Black tree 25% Allele black 25% Brown tree 50% Allele brown 50% White tree 25% Allele white 25% After one generation, allele frequency remains stable (between 48% and 52%) and the genotype frequency becomes predominantly brown. This is because there is always the presence of black and white genotypes which cause slight variation. If you change the selection of trees to black 10%, white 10%, brown 80%, almost identical results occur (between 49% and 51% variation in allele frequency = stabilized). Q 4.3) To show diversifying selection we set the tree types to 45% black, 45% white and 10% brown. Genotype frequency by the 1st generation, there was a large decrease in brown morphs of moth and the equivalent increase in black and white morphs. This continues till F5 when the black morph became slightly more dominant (on average) due to random mating. The brown morph was phased out by F18 (on average) on most of the 100 tree stands. All alleles become fixed for either black or white by F23 (49% white, 51% black) (Fig 13). Figure 13. Shows 50% black and 50% white showing a 1:1 ratio Q 4.4) Small differences in fitness are effective in changing allele frequencies. Small differences in fitness have proportionally slower rates of allele frequency change compared with large differences in fitness. We conducted additional experiments with varying proportions of tree types. The results are as follows: (Where stand size = 4000, number of stands = 100, allele frequencies = white 20%, brown 60%, black 20%, tree frequencies = white 32%, brown 32%, black 36%). Even though there are a lower proportion of black alleles (A) to begin with, those alleles will have a higher fitness than white or brown as there is a higher percentage of black tree types. Over time this will equate to an increase in black morphs. There is, however, a Catherine Carrick 200884273 point where even if the black allele is fittest but there isnt a high enough population in the first place, it will crash and not recover. Q 4.5) Genetic variation is maintained under balancing selection because the allele frequencies remain stable. There is no fixation (presuming the all mating is random). The heterozygote allele is favoured and thus balances the homozygous allele. Assignment 5: Migration Q 5.1) Migration counteracts the effects of genetic drift. (Fig 14 and 15) Figure 14. Stand size of 500 and no migration shows heterozygosity varying over all populations. Green line = average heterozygosity over all populations. Figure 15. Shows stand size 500, and 8% migration. Shows migration maintains heterozygosity and there is less deviation from the average (green line) Catherine Carrick 200884273 Assignment 6: Population Bottlenecks Q 6.1) Disaster led to the loss of alleles and reduced heterozygosity. The more disasters there where, the more decreased the diversity became. (Fig 16, 17, 18) Figure 16. Control condition Shows low drift conferred by high population sizes (4000), all other variables adjusted to give Hardy-Weinberg equilibrium. Figure 17. Disaster parameters set to sometimes as opposed to never. Individual populations prone to fixation and loss of alleles. Catherine Carrick 200884273 Figure 18. Disaster frequency set to often rather than sometimes. Loss of diversity occurs faster than in figure 13 with most populations losing one or the other allele by generation 80. Q 6.2) Disaster increased the rate of extinction. The more regular the disaster, the more extinctions. Q 6.3) Migration moderated the effect that disasters had on the population. Discussion The results of our experiments clearly show that genetic drift effects smaller populations where heterozygoisity is lost rapidly and as the carrying capacity decreases, the risk of extinction increases. The is because the proportion of individuals with a certain phenotype within a small population are largely influenced by random variation in survival, and over time, the change in proportion of genotypes in subsequent generations leads to genetic drift. If one was to aim to conserve a hypothetical species, one would expect that because it is endangered, it would be a small population. To maintain genetic diversity among this species, one would need a large enough breeding population to begin with. Unfortunately, as is the case with most endangered species, populations become geographically isolated, mainly due to human disruption of habitat. Migration between breeding populations decreases and they become fragmented. Conservation efforts may be due to natural disasters such as tsunam is, fires etc, but are mainly to prevent the constant onslaught of human activities such as illegal logging in conservation areas. Figure 17 illustrates the effects of a bottleneck following a disaster, showing reduced variability (and a small population) leading to loss and/or fixation of alleles. As with genetic drift, the way to prevent population crashes, or rather soften the effects of bottlenecks, is to encourage migration among populations. This can be achieved by implementing the protection of corridors between known endangered populations. In theory, the populations can migrate between areas, maintaining a high enough level of breeding and genetic variation, to counter the effects of inbreeding depression or genetic drift (Fig 14 and 15). An example of how corridors may re-connect fragmented populations can be seen in Bhutans Jigme Singye Wangchuck National Park (www.panthera.org). Catherine Carrick 200884273 Figure 19. Map of known tiger populations (red) and proposed tiger corridors (orange). Data taken from www.panthera.org The proposed Eastern Himalayan corridor may help towards connecting isolated populations of tigers, and thus increasing genetic diversity (if these populations successfully reproduce with one another) (Fig 19). Random mating, as apposed to assortative mating, will increase heterozygosity and stabilise a population (Fig 10). This acts against genetic drift and stops the population form diverging as quickly. In a hypothetical situation then, you would preferably allow mating to occur naturally and at random. However, some conservation efforts include that of translocation of individuals or cross breeding certain individuals from separate populations. For this to be advantageous to the species, one must consider maintaining genetic diversity by genotyping the individuals before translocation. It would be senseless to swap or breed an AA individual with another AA individual from a separate population as this would lead to loss or fixation and not increase diversity. Our studies with B. betularia in question 4 to 4.5 show that intermediates are favoured over extreme phenotypes and that genetic variation is maintained under balancing (stabilizing) selection because the allele frequencies remain stable. There is no fixation (presuming the all mating is random). The heterozygote allele is favoured and thus balances the homozygous allele. As well as considering the genetic diversity of a species and its genealogy, one must understand the species by means of observations in the field including its behaviour. Later studies of B. betularia reinforced the need for such observations as it was found to rest high in the branches rather than on the trunks of trees as previously calculated. Also, modern science allowed for the study of its UV qualities which had otherwise been unaccounted for when considering levels of predation by birds. A close study of mating patterns should ideally be assessed to ensure the outcome of migration; corridors, translocation etc will be advantageous in terms of fitness. Catherine Carrick 200884273

Narrative Essays: To Tell a Story Essay

There are four types of essays: Exposition – gives information about various topics to the reader. Description – describes in detail characteristics and traits. Argument – convinces the reader by demonstrating the truth or falsity of a topic. Narrative – tells a story, usually from one person’s viewpoint. A narrative essay uses all the story elements – a beginning and ending, plot, characters, setting and climax – all coming together to complete the story. Essential Elements of Narrative Essays The focus of a narrative essay is the plot, which is told using enough details to build to a climax. Here’s how: It is usually told chronologically. It usually has a purpose, which is usually stated in the opening sentence. It may use dialogue.  It is written with sensory details and vivid descriptions to involve the reader. All these details relate in some way to the main point the writer is making. All of these elements need to seamlessly combine. A few examples of narrative essays follow. Narrative essays can be quite long, so instead of a full length example of an entire essay, only the beginnings of essays are included: Learning Can Be Scary This excerpt about learning new things and new situations is an example of a personal narrative essay that describes learning to swim. â€Å"Learning something new can be a scary experience. One of the hardest things I’ve ever had to do was learn how to swim. I was always afraid of the water, but I decided that swimming was an important skill that I should learn. I also thought it would be good exercise and help me to become physically  stronger. What I didn’t realize was that learning to swim would also make me a more confident person. New situations always make me a bit nervous, and my first swimming lesson was no exception. After I changed into my bathing suit in the locker room, I stood timidly by the side of the pool waiting for the teacher and other students to show up. After a couple of minutes the teacher came over. She smiled and introduced herself, and two more students joined us. Although they were both older than me, they didn’t seem to be embarrassed about not knowing how to swim. I began to feel more at ease.† The Manager. The Leader. The following excerpt is a narrative essay from a story about a manager who was a great leader. Notice the intriguing first sentence that captures your attention right away. â€Å"Jerry was the kind of guy you love to hate. He was always in a good mood and always had something positive to say. When someone would ask him how he was doing, he would reply, â€Å"If I were any better, I would be twins!† He was a unique manager because he had several waiters who had followed him around from restaurant to restaurant. The reason the waiters followed Jerry was because of his attitude. He was a natural motivator. If an employee was having a bad day, Jerry was there telling the employee how to look on the positive side of the situation.† The Climb This excerpt from the climb also captures your attention right away by creating a sense of mystery. The reader announces that he or she has â€Å"this fear† and you want to read on to see what that fear is. â€Å"I have this fear. It causes my legs to shake. I break out in a cold sweat. I start jabbering to anyone who is nearby. As thoughts of certain death run through my mind, the world appears a precious, treasured place. I imagine my own funeral, then shrink back at the implications of where my thoughts are taking me. My stomach feels strange. My palms are clammy. I am terrified of heights.Of course, it’s not really a fear of being in a high place. Rather, it is the view of a long way to fall, of rocks far below me and no firm wall between me and the edge. My sense of security is screamingly absent. There  are no guardrails, flimsy though I picture them, or other safety devices. I can rely only on my own surefootedness—or lack thereof.† Disney Land The following narrative essay involves a parent musing about taking her kids to Disney Land. â€Å"It was a hot sunny day, when I finally took my kids to the Disney Land. My son Matthew and my daughter Audra endlessly asked me to show them the dream land of many children with Mickey Mouse and Snow-white walking by and arousing a huge portion of emotions. Somehow these fairy tale creatures can make children happy without such â€Å"small† presents as $100 Lego or a Barby’s house in 6 rooms and garden furniture. Therefore, I thought that Disney Land was a good invention for loving parents.† The Sacred Grove of Oshogbo by Jeffrey Tayler The following essay contains descriptive language that helps to paint a vivid picture for the reader of an encounter with a man. â€Å"As I passed through the gates I heard a squeaky voice. A diminutive middle-aged man came out from behind the trees — the caretaker. He worked a toothbrush-sized stick around in his mouth, digging into the crevices between algae’d stubs of teeth. He was barefoot; he wore a blue batik shirt known as a buba, baggy purple trousers, and an embroidered skullcap. I asked him if he would show me around the shrine. Motioning me to follow, he spat out the results of his stick work and set off down the trail.† Playground Memory The first excerpt from, â€Å"Playground Memory†, has very good sensory details. â€Å"Looking back on a childhood filled with events and memories, I find it rather difficult to pick on that leaves me with the fabled â€Å"warm and fuzzy feelings.† As the daughter of an Air Force Major, I had the pleasure of traveling across America in many moving trips. I have visited the monstrous trees of the Sequoia National Forest, stood on the edge of the Grande Canyon and have jumped on the beds at Caesar’s Palace in Lake Tahoe. However, I  have discovered that when reflecting on my childhood, it is not the trips that come to mind, instead there are details from everyday doings; a deck of cards, a silver bank or an ice cream flavor. One memory that comes to mind belongs to a day of no particular importance. It was late in the fall in Merced, California on the playground of my old elementary school; an overcast day with the wind blowing strong. I stood on the blacktop, pulling my hoodie over my ears. The wind was causing miniature tornados; we called them â€Å"dirt devils†, to swarm around me.† Christmas Cookies The second of the two narrative essay examples is an excerpt from â€Å"Christmas Cookies.† â€Å"Although I have grown up to be entirely inept at the art of cooking, as to make even the most wretched chef ridicule my sad baking attempts, my childhood would have indicated otherwise; I was always on the countertop next to my mother’s cooking bowl, adding and mixing ingredients that would doubtlessly create a delicious food. When I was younger, cooking came intrinsically with the holiday season, which made that time of year the prime occasion for me to unite with ounces and ounces of satin dark chocolate, various other messy and gooey ingredients, numerous cooking utensils, and the assistance of my mother to cook what would soon be an edible masterpiece. The most memorable of the holiday works of art were our Chocolate Crinkle Cookies, which my mother and I first made when I was about six and are now made annually.† Ads by Google Online Screenwriting Award Winning Instructor. Includes Free One-on-One Consult. www.writeyourscreenplay.com Salvation-How God Saves How our Creator reconciles with us through his great sacrifice www.godsavesus.com Tips on Writing a Narrative Essay When writing a narrative essay, remember that you are sharing sensory and emotional details with the reader. Your words need to be vivid and colorful to help the reader feel the same feelings that you felt. Elements of the story need to support the point you are making and you need to remember to make reference to that point in the first sentence. You should make use of conflict and sequence like in any story. You may use flashbacks and flash forwards to help the story build to a climax. It is usually written in the first person, but third person may also be used. Remember, a well-written narrative essay tells a story and makes a point.

George Orwell’s Animal Farm Conflicts Essay

In this controlled assessment I am going to explain in my own words and by using quotes from the book how George Orwell refers to at least three conflicts in the fable Animal Farm. George Orwell wrote the controversial book Animal Farm, and very nearly didn’t get it published. But in 1945 Secker and Warburg published the book and has since become one of the most read and talked about books of our time. Orwell tells his story which refers to the Russian revolution by using an allegory form of text. Orwell’s book can be read and interpreted on at least two different levels of understanding. 1) Being a fable, which children would hear how animals have taken over the farm and are all living, working and singing together ect. Or the 2nd) in which adults would read it and in most cases, working class family’s would be able to relate to the theme of the book. In the book George Orwell explains to the reader how one of the main characters Old Major (who represents in rea l life Vladimir lenin and karl marx.) who is â€Å"the prize middle white boar† and the most respected and knowledgeable animal on the farm has had a dream. Old Major organises a meeting with all the other farm animals in the barn that night. He explains to all the animals the dream he has had and how they would all be better off without the humans (Mr Jones) Old Major goes on to say â€Å"man is the only real enemy we have. Remove man from the scene, and the root cause of hunger and overwork is abolished forever† Three nights later Old Major dies and we are introduced to the pigs! â€Å"who were generally recognised as being the cleverest of the animals† the pigs took over the running of the farm animals which soon started to show signs of tension between the two main characters Napoleon and Snowball who are portraying Stalin and Trotsky. Orwell shows us several different conflicts between the two pigs, regardless of the fact that they are both working on getting a better kind of life for themselves and the other animals. Or so they make out to be. However soon after the rebellion had formed and â€Å"jones was expelled† the relationship between Napoleon and Snowball begins to worsen. Consequently the two farm dogs had just had a litter of pups which Napoleon had taken away soon after they had been born, to secretly train them to become his own private guard dogs. After constantly feeling like he is losing his role as leader, Napoleon sets the â€Å"fierce dogs† on Snowball who chase him of the farm and is never to be seen again. Orwell uses the adjective word â€Å"fierce† in this part of the story to emphasize to the reader just how much tension there has been between the two pigs, He uses connotations and adjectives such as â€Å"enormous dogs wearing brass studded collars† to describe the size and look of the dogs chasing Snowball, Therefor leaving Napoleon solely in charge. Soon after this the animals begin to build a windmill which Snowball had originally planned, but with Snowball being gone N apoleon took it upon himself to portray it as his own. Here Orwell tells us of another form of conflict beginning by using connotations such as â€Å"slaves† to describe how hard the Animals have been working instead of using words like hard or more than usual! The animals work hard all year and often go with not nearly enough food but yet still do not complain. The winter that year is hard and Orwell uses short and simple sentences such as â€Å"November came with raging south west winds† to build an atmosphere before using verbs such as â€Å"violent† to describe the winds before the reader goes on to read that there was a terrible storm and the windmill is blown down while only half way built. Napoleon automatically blames this on Snowball and additionally everything else that goes wrong too. Napoleon constantly goes on about things that are going wrong so that the other animals also start to believe that Snowball is the one doing it. Further to this, another less tangible conflict is that between appearances and the reality all throughout the book the pigs manipulate the commandments to justify Napoleon’s behaviour. For example, Squealer (who represents propaganda) persuades the other animals that Snowball actually lead the humans to the farm to have what is now named the â€Å"battle of the cowshed†. The â€Å"battle of the cowshed† was fought and won by the animals at the side of the barn where the commandments are written on, and have over time gradually been winded down to one â€Å"all animals are equal, but some are more equal than others† The animals can never quiet remember if or when they have been changed so just assume that they have not and they have always been this was from the beginning.

Blogging And Social Networking...The New World Order Essay

Is giving people what they want making happiness or argument in the end? Using the internet has started becoming a popular way of getting information and staying in contact with friends and family, but is blogging and social networking a way to stay in touch or a â€Å"way to BE the news.† Blogging is becoming so popular some are worried that it is widening the gray area between right and wrong/ fact and fiction. On the pro blogger side some say they do not publish anything that would get them sued or that they did not research thoroughly. Blogs are being used to speak on everything the main stream media is afraid to say. â€Å"The Cult of an Amateur† is an essay giving the opinion that blogging and internet sources like Wikipedia, YouTube,†¦show more content†¦Comparing the growth of mainstream blogging as a positive move into the future verses seeing the situation as amateurs putting their opinion for the world to see as fact all over the internet is like comparing a dell computer to a Mac both have their ups and downs. Yes i t is true anyone can their opinion post it on the internet and someone else may see it as fact but it is not far to say about everyone. Also when doing research and trying to find answers the researchers’ job is to seek out the creditability of the article and even get other opinions from different sites. 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